Ontology highlight
ABSTRACT:
SUBMITTER: Morgan AH
PROVIDER: S-EPMC3678246 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Nature protocols 20101110 12
Phospholipid-esterified oxylipins include newly described families of bioactive lipids generated by lipoxygenases in immune cells. Until now, assays for their quantitation were not well developed or widely available. Here, we describe a mass spectrometric protocol that enables accurate measurement of several esterified oxylipins--in particular hydro(pero)xyeicosatetraenoic acids, hydroxyoctadecadienoic acids, hydroxydocosahexaenoic acids and keto-eicosatetraenoic acids--attached to either phosph ...[more]