Ontology highlight
ABSTRACT:
SUBMITTER: Wroblewska A
PROVIDER: S-EPMC3680040 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Wroblewska Aleksandra A van Haren Simon D SD Herczenik Eszter E Kaijen Paul P Ruminska Aleksandra A Jin Sheng-Yu SY Zheng X Long XL van den Biggelaar Maartje M ten Brinke Anja A Meijer Alexander B AB Voorberg Jan J
Blood 20120412 22
Development of neutralizing Abs to blood coagulation factor VIII (FVIII) provides a major complication in hemophilia care. In this study we explored whether modulation of the uptake of FVIII by APCs can reduce its intrinsic immunogenicity. Endocytosis of FVIII by professional APCs is significantly blocked by mAb KM33, directed toward the C1 domain of FVIII. We created a C1 domain variant (FVIII-R2090A/K2092A/F2093A), which showed only minimal binding to KM33 and retained its activity as measured ...[more]