Unknown

Dataset Information

0

Dengue-specific subviral nanoparticles: design, creation and characterization.


ABSTRACT:

Background

Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.

Methods

We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.

Results

The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.

Conclusions

VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.

SUBMITTER: Khetarpal N 

PROVIDER: S-EPMC3680219 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

altmetric image

Publications


<h4>Background</h4>Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutraliz  ...[more]

Similar Datasets

| S-EPMC4586464 | biostudies-literature
| S-EPMC3116464 | biostudies-literature
| S-EPMC4145673 | biostudies-literature
| S-EPMC6929274 | biostudies-literature
| S-EPMC8791083 | biostudies-literature
| S-EPMC2790604 | biostudies-literature
| S-EPMC5625772 | biostudies-literature
| S-EPMC5931519 | biostudies-literature
| S-EPMC8080695 | biostudies-literature
| S-EPMC8137585 | biostudies-literature