Ontology highlight
ABSTRACT: Background
Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods
We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results
The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions
VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
SUBMITTER: Khetarpal N
PROVIDER: S-EPMC3680219 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Khetarpal Niyati N Poddar Ankur A Nemani Satish K SK Dhar Nisha N Patil Aravind A Negi Priyanka P Perween Ashiya A Viswanathan Ramaswamy R Lünsdorf Heinrich H Tyagi Poornima P Raut Rajendra R Arora Upasana U Jain Swatantra K SK Rinas Ursula U Swaminathan Sathyamangalam S Khanna Navin N
Journal of nanobiotechnology 20130525
<h4>Background</h4>Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutraliz ...[more]