Ontology highlight
ABSTRACT:
SUBMITTER: Rho JH
PROVIDER: S-EPMC3680356 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Rho Jung-hyun JH Lampe Paul D PD
Journal of proteome research 20130329 5
We report on a novel, high-dimensional method to detect autoantibodies that are complexed with their natural autoantigens. Specifically, autoantibody-autoantigen complexes in serum or plasma are directly incubated onto a high-density antibody microarray. Detection of the bound autoantibody-antigen complex is made via fluorescently labeled antihuman immunoglobulin G or other immunoglobulin isotype secondary antibodies and quantification in a microarray scanner. Uncomplexed antibodies do not inter ...[more]