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Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping.


ABSTRACT: We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbA?R+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

SUBMITTER: Yeh HH 

PROVIDER: S-EPMC3681297 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping.

Yeh Hsu-Hua HH   Chang Shu-Lin SL   Chiang Yi-Ming YM   Bruno Kenneth S KS   Oakley Berl R BR   Wu Tung-Kung TK   Wang Clay C C CC  

Organic letters 20130131 4


We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the prod  ...[more]

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