TALEN-mediated editing of the mouse Y chromosome.
Ontology highlight
ABSTRACT:
SUBMITTER: Wang H
PROVIDER: S-EPMC3681814 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Publications
TALEN-mediated editing of the mouse Y chromosome.
Wang Haoyi H Hu Yueh-Chiang YC Markoulaki Styliani S Welstead G Grant GG Cheng Albert W AW Shivalila Chikdu S CS Pyntikova Tatyana T Dadon Daniel B DB Voytas Daniel F DF Bogdanove Adam J AJ Page David C DC Jaenisch Rudolf R
Nature biotechnology 20130512 6
The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes--Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome. ...[more]
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