Ontology highlight
ABSTRACT:
SUBMITTER: Sripa J
PROVIDER: S-EPMC3682765 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Sripa Jittiyawadee J Pinlaor Porntip P Brindley Paul J PJ Sripa Banchob B Kaewkes Sasithorn S Robinson Mark W MW Young Neil D ND Gasser Robin B RB Loukas Alex A Laha Thewarach T
Parasitology international 20110501 3
Functional genomics have not been reported for Opisthorchis viverrini or the related fish-borne fluke, Clonorchis sinensis. Here we describe the introduction by square wave electroporation of Cy3-labeled small RNA into adult O. viverrini worms. Adult flukes were subjected to square wave electroporation employing a single pulse for 20 ms of 125V in the presence of 50 μg/ml of Cy3-siRNA. The parasites tolerated this manipulation and, at 24 and 48 h after electroporation, fluorescence from the Cy3- ...[more]