Unknown

Dataset Information

0

Bead assembly magnetorotation as a signal transduction method for protein detection.


ABSTRACT: This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1 ?m magnetic beads into a bead assembly, formed at the bottom of a 1 ?L hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field.

SUBMITTER: Hecht A 

PROVIDER: S-EPMC3683359 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Bead assembly magnetorotation as a signal transduction method for protein detection.

Hecht Ariel A   Commiskey Patrick P   Shah Nicholas N   Kopelman Raoul R  

Biosensors & bioelectronics 20130406


This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1 μm magnetic beads into a bead assembly, formed at the bottom of a 1 μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in  ...[more]

Similar Datasets

| S-EPMC5036152 | biostudies-literature
2022-03-03 | GSE162461 | GEO
2006-07-28 | E-TABM-98 | biostudies-arrayexpress
| S-EPMC2868067 | biostudies-literature
| S-EPMC6539988 | biostudies-literature
| S-EPMC3351598 | biostudies-literature
| S-EPMC2910557 | biostudies-literature
| S-EPMC6403535 | biostudies-literature
| S-EPMC7156525 | biostudies-literature
| S-EPMC4188267 | biostudies-literature