Differential expression of Desulfovibrio vulgaris genes in response to Cu(II) and Hg(II) toxicity.
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SUBMITTER: Chang IS
PROVIDER: S-EPMC368381 | biostudies-literature | 2004 Mar
REPOSITORIES: biostudies-literature
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Differential expression of Desulfovibrio vulgaris genes in response to Cu(II) and Hg(II) toxicity.
Chang In Seop IS Groh Jennifer L JL Ramsey Matthew M MM Ballard Jimmy D JD Krumholz Lee R LR
Applied and environmental microbiology 20040301 3
The response of Desulfovibrio vulgaris to Cu(II) and Hg(II) was characterized. Both metals increased the lag phase, and Cu(II) reduced cell yield at concentrations as low as 50 microM. mRNA expression was analyzed using random arbitrarily primed PCR, differential display, and quantitative PCR. Both Cu(II) and Hg(II) (50 micro M) caused upregulation of mRNA expression for an ATP binding protein (ORF2004) and an ATPase (ORF856) with four- to sixfold increases for Hg(II) and 1.4- to 3-fold increase ...[more]
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