Dataset Information
L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.
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ABSTRACT:
SUBMITTER: van der Kaaij H
PROVIDER: S-EPMC368417 | biostudies-literature | 2004 Mar
REPOSITORIES: biostudies-literature
Publications
L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.
Applied and environmental microbiology 20040301 3
Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase. ...[more]
