Unknown

Dataset Information

0

Microstructural remodeling of articular cartilage following defect repair by osteochondral autograft transfer.


ABSTRACT: To assess collagen network alterations occurring with flow and other abnormalities of articular cartilage at medial femoral condyle (MFC) sites repaired with osteochondral autograft (OATS) after 6 and 12 months, using quantitative polarized light microscopy (qPLM) and other histopathological methods.The collagen network structure of articular cartilage of OATS-repaired defects and non-operated contralateral control sites were compared by qPLM analysis of parallelism index (PI), orientation angle (?) relative to the local tissue axes, and retardance (?) as a function of depth. qPLM parameter maps were also compared to ICRS and Modified O'Driscoll grades, and cell and matrix sub-scores, for sections stained with H&E and Safranin-O, and for Collagen-I and II.Relative to non-operated normal cartilage, OATS-repaired regions exhibited structural deterioration, with low PI and more horizontal ?, and unique structural alteration in adjacent host cartilage: more aligned superficial zone, and reoriented deep zone lateral to the graft, and matrix disorganization in cartilage overhanging the graft. Shifts in ? and PI from normal site-specific values were correlated with histochemical abnormalities and co-localized with changes in cell organization/orientation, cloning, or loss, indicative of cartilage flow, remodeling, and deterioration, respectively.qPLM reveals a number of unique localized alterations of the collagen network in both adjacent host and implanted cartilage in OATS-repaired defects, associated with abnormal chondrocyte organization. These alterations are consistent with mechanobiological processes and the direction and magnitude of cartilage strain.

SUBMITTER: Raub CB 

PROVIDER: S-EPMC3684698 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Microstructural remodeling of articular cartilage following defect repair by osteochondral autograft transfer.

Raub C B CB   Hsu S C SC   Chan E F EF   Shirazi R R   Chen A C AC   Chnari E E   Semler E J EJ   Sah R L RL  

Osteoarthritis and cartilage 20130322 6


<h4>Objective</h4>To assess collagen network alterations occurring with flow and other abnormalities of articular cartilage at medial femoral condyle (MFC) sites repaired with osteochondral autograft (OATS) after 6 and 12 months, using quantitative polarized light microscopy (qPLM) and other histopathological methods.<h4>Design</h4>The collagen network structure of articular cartilage of OATS-repaired defects and non-operated contralateral control sites were compared by qPLM analysis of parallel  ...[more]

Similar Datasets

| S-EPMC5123990 | biostudies-literature
| S-EPMC7695613 | biostudies-literature
| S-EPMC5452095 | biostudies-other
| S-EPMC7823117 | biostudies-literature
| S-EPMC7917345 | biostudies-literature
| S-EPMC7933472 | biostudies-literature
| S-EPMC5754326 | biostudies-literature
| S-EPMC9069659 | biostudies-literature
| S-EPMC5689111 | biostudies-literature
| S-EPMC6713909 | biostudies-other