Ontology highlight
ABSTRACT:
SUBMITTER: Vega Y
PROVIDER: S-EPMC3685689 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Vega Yolanda Y Delgado Elena E Carrera Cristina C Nebreda Paloma P Fernández-García Aurora A Cuevas María Teresa MT Pérez-Álvarez Lucía L Thomson Michael M MM
AIDS research and human retroviruses 20130508 7
We analyzed RNA splice site usage in three HIV-1 subtype B primary isolates through reverse transcriptase polymerase chain reaction (RT-PCR) amplification of spliced RNAs using a fluorescently labeled primer, with computerized size determination and quantification of PCR products, which were also identified by clone sequencing. In one isolate, P2149-3, unusual and unreported spliced transcripts were detected. This isolate preferentially used for rev RNA generation a 3' splice site (3'ss) located ...[more]