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ABSTRACT:
SUBMITTER: Tormos JR
PROVIDER: S-EPMC3685723 | biostudies-literature | 2005 Oct
REPOSITORIES: biostudies-literature
Tormos Jose R JR Wiley Kenneth L KL Seravalli Javier J Nachon Florian F Masson Patrick P Nicolet Yvain Y Quinn Daniel M DM
Journal of the American Chemical Society 20051001 42
Secondary beta-deuterium kinetic isotope effects have been measured as a function of substrate concentration for recombinant human butyrylcholinesterase-catalyzed hydrolysis of acetyl-L3-thiocholine (L = 1H or 2H). The isotope effect on V/K is inverse, D3V/K = 0.93 +/- 0.03, which is consistent with conversion of the sp2 hybridized carbonyl carbon of the scissile ester bond of the E + A reactant state to a quasi-tetrahedral structure in the acylation transition state. In contrast, the isotope ef ...[more]