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Submillisecond elastic recoil reveals molecular origins of fibrin fiber mechanics.


ABSTRACT: Fibrin fibers form the structural scaffold of blood clots. Thus, their mechanical properties are of central importance to understanding hemostasis and thrombotic disease. Recent studies have revealed that fibrin fibers are elastomeric despite their high degree of molecular ordering. These results have inspired a variety of molecular models for fibrin's elasticity, ranging from reversible protein unfolding to rubber-like elasticity. An important property that has not been explored is the timescale of elastic recoil, a parameter that is critical for fibrin's mechanical function and places a temporal constraint on molecular models of fiber elasticity. Using high-frame-rate imaging and atomic force microscopy-based nanomanipulation, we measured the recoil dynamics of individual fibrin fibers and found that the recoil was orders of magnitude faster than anticipated from models involving protein refolding. We also performed steered discrete molecular-dynamics simulations to investigate the molecular origins of the observed recoil. Our results point to the unstructured ?C regions of the otherwise structured fibrin molecule as being responsible for the elastic recoil of the fibers.

SUBMITTER: Hudson NE 

PROVIDER: S-EPMC3686331 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Submillisecond elastic recoil reveals molecular origins of fibrin fiber mechanics.

Hudson Nathan E NE   Ding Feng F   Bucay Igal I   O'Brien E Timothy ET   Gorkun Oleg V OV   Superfine Richard R   Lord Susan T ST   Dokholyan Nikolay V NV   Falvo Michael R MR  

Biophysical journal 20130601 12


Fibrin fibers form the structural scaffold of blood clots. Thus, their mechanical properties are of central importance to understanding hemostasis and thrombotic disease. Recent studies have revealed that fibrin fibers are elastomeric despite their high degree of molecular ordering. These results have inspired a variety of molecular models for fibrin's elasticity, ranging from reversible protein unfolding to rubber-like elasticity. An important property that has not been explored is the timescal  ...[more]

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