Ontology highlight
ABSTRACT:
SUBMITTER: Kee JM
PROVIDER: S-EPMC3686892 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Kee Jung-Min JM Oslund Rob C RC Perlman David H DH Muir Tom W TW
Nature chemical biology 20130526 7
Despite its importance in central metabolism and bacterial cell signaling, protein histidine phosphorylation has remained elusive with respect to its extent and functional roles in biological systems because of the lack of adequate research tools. We report the development of the first pan-phosphohistidine (pHis) antibody using a stable pHis mimetic as the hapten. This antibody was successfully used in ELISA, western blotting, dot blot assays and immunoprecipitation and in detection and identifi ...[more]