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Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report.


ABSTRACT: Cerebral angiostrongyliasis is an acute inflammation caused by the infection of the nematode Angiostrongylus cantonensis that results in eosinophilic meningitis. The current immunological assay of choice is an immunoblot that detects antibodies to a 31 kDa protein present in crude extracts of the female worm. Recently we have identified diagnostic targets from excretion and secretion products and determined the composition of the 31 kDa antigen after 2-D gel electrophoresis and mass spectrometry. Here we cloned and expressed five proteins in prokaryotic and eukaryotic systems. Recombinant proteins were purified and analysed by Western blot assays and among them 14-3-3, Lec5 and ES7 were recognized by Angiostrongylus-specific serum, although the signal was weak.

SUBMITTER: Morassutti AL 

PROVIDER: S-EPMC3689479 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report.

Morassutti Alessandra L AL   Perelygin Andrey A   Levert Keith K   Lin Seh-Ching SC   Lee Yeuk-Mui YM   da Silva Alexandre J AJ   Wilkins Patricia P PP   Graeff-Teixeira Carlos C  

Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health 20130601 6 Suppl 2


Cerebral angiostrongyliasis is an acute inflammation caused by the infection of the nematode Angiostrongylus cantonensis that results in eosinophilic meningitis. The current immunological assay of choice is an immunoblot that detects antibodies to a 31 kDa protein present in crude extracts of the female worm. Recently we have identified diagnostic targets from excretion and secretion products and determined the composition of the 31 kDa antigen after 2-D gel electrophoresis and mass spectrometry  ...[more]

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