Time-lapse imaging of self- and cross-pollinations in Brassica rapa.

ABSTRACT:

Background and aims

Pollination is an important process in the life cycle of plants and is the first step in bringing together the male and female gametophytes for plant reproduction. While pollination has been studied for many years, accurate knowledge of the morphological aspects of this process is still far from complete. This study therefore focuses on a morphological characterization of pollination, using time-series image analysis of self- and cross-pollinations in Brassica rapa.

Methods

Time-lapse imaging of pollen behaviour during self- and cross-pollinations was recorded for 90 min, at 1 min intervals, using a stereoscopic microscope. Using time-series digital images of pollination, characteristic features of pollen behaviours during self- and cross-pollinations wer

SUBMITTER: Hiroi K 

PROVIDER: S-EPMC3690991 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature