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Development of rapid microwave-mediated and low-temperature bacterial transformations.

ABSTRACT: The introduction of exogenous DNA into Escherichia coli is a cornerstone of molecular biology. Herein, we investigate two new mechanisms for bacterial transformation involving either the use of microwave irradiation or a freeze-thaw protocol in liquid nitrogen. Ultimately, both methods afforded successful transfer of plasmid DNA into bacterial cells, with the freeze-thaw technique yielding efficiencies of ~10(5). More importantly, both techniques effectively eliminated the need for the preparation of competent cells.

SUBMITTER: Tripp VT

PROVIDER: S-EPMC3691398 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Development of rapid microwave-mediated and low-temperature bacterial transformations.

Tripp Valerie T VT Maza Johnathan C JC Young Douglas D DD

Journal of chemical biology 20130521 3

The introduction of exogenous DNA into Escherichia coli is a cornerstone of molecular biology. Herein, we investigate two new mechanisms for bacterial transformation involving either the use of microwave irradiation or a freeze-thaw protocol in liquid nitrogen. Ultimately, both methods afforded successful transfer of plasmid DNA into bacterial cells, with the freeze-thaw technique yielding efficiencies of ~10(5). More importantly, both techniques effectively eliminated the need for the preparati ...[more]

PMID: 24432129

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