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Disruption of the murine Ap2?1 gene causes nonsyndromic cleft palate.


ABSTRACT: Development of the secondary palate in mammals is a complex process that can be easily perturbed, leading to the common and distressing birth defect cleft palate. Animal models are particularly useful tools for dissecting underlying genetic components of cleft palate. We describe a new cleft palate model resulting from a transgene insertion mutation. Transgene insertional mutagenesis disrupts the genomic organization and expression of the Ap2?1 gene located on chromosome 11. This gene encodes the ?2-adaptin subunit of the heterotetrameric adaptor protein 2 complex involved in clathrin-dependent endocytosis. Homozygous cleft palate mutant mice express no Ap2?1 messenger RNA or ?2-adaptin protein and die during the perinatal period. Heterozygous mice are phenotypically normal despite expressing diminished ?2-adaptin messenger RNA and protein compared with wildtype. Remarkably, the paralogous ?1-adaptin subunit of the adaptor protein 1 complex partially substitutes for the missing ?2-adaptin in embryonic fibroblasts from homozygous mutant mice, resulting in assembly of reduced levels of an adaptor protein 2 complex bearing ?1-adaptin. This variant adaptor protein 2 complex is, therefore, apparently capable of maintaining viability of the homozygous mutant embryos until birth but insufficient to support palatogenesis. Nonsyndromic cleft palate in an animal model is associated with disruption of the Ap2?1 gene.

SUBMITTER: Li W 

PROVIDER: S-EPMC3691559 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Disruption of the murine Ap2β1 gene causes nonsyndromic cleft palate.

Li Wei W   Puertollano Rosa R   Bonifacino Juan S JS   Overbeek Paul A PA   Everett Eric T ET  

The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association 20100302 6


Development of the secondary palate in mammals is a complex process that can be easily perturbed, leading to the common and distressing birth defect cleft palate. Animal models are particularly useful tools for dissecting underlying genetic components of cleft palate. We describe a new cleft palate model resulting from a transgene insertion mutation. Transgene insertional mutagenesis disrupts the genomic organization and expression of the Ap2β1 gene located on chromosome 11. This gene encodes th  ...[more]

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