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Multicolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexes.

ABSTRACT: Many interesting and important membrane proteins are hetero-oligomeric. However, besides naturally abundant examples, the structures of relatively few such complexes are known. Partly, this is due to difficulties in expression, stoichiometric assembly, and in the evaluation of their stability prior to crystallization trials. Here we describe a new approach, which allows rapid assessment of protein complex quality, assembly and stoichiometry, simplifying the search for conditions conducive to long-term stability and crystallization. Multicolour fluorescence size-exclusion chromatography (MC-FSEC) is used to enable tracking of individual subunits through expression, solubilization and purification steps. We show how the method has been applied to the heterodimeric transporter associated with antigen processing (TAP) and demonstrate how it may be extended in order to analyse membrane multisubunit assemblies.

SUBMITTER: Parcej D

PROVIDER: S-EPMC3692423 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Multicolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexes.

Parcej David D Guntrum Renate R Schmidt Sabine S Hinz Andreas A Tampé Robert R

PloS one 20130625 6

Many interesting and important membrane proteins are hetero-oligomeric. However, besides naturally abundant examples, the structures of relatively few such complexes are known. Partly, this is due to difficulties in expression, stoichiometric assembly, and in the evaluation of their stability prior to crystallization trials. Here we describe a new approach, which allows rapid assessment of protein complex quality, assembly and stoichiometry, simplifying the search for conditions conducive to lon ...[more]

PMID: 23825631

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