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Alternative Splicing of the Amelogenin Gene in a Caudate Amphibian, Plethodon cinereus.


ABSTRACT: As the major enamel matrix protein contributing to tooth development, amelogenin has been demonstrated to play a crucial role in tooth enamel formation. Previous studies have revealed amelogenin alternative splicing as a mechanism for amelogenin heterogeneous expression in mammals. While amelogenin and its splicing forms in mammalian vertebrates have been characterized, splicing variants of amelogenin gene still remains largely unknown in non-mammalian species. Here, using PCR and sequence analysis we discovered two novel amelogenin transcript variants in tooth organ extracts from a caudate amphibian, the salamander Plethodoncinereus. The one was shorter -S- (416 nucleotides including untranslated regions, 5 exons) and the other larger -L- (851 nt, 7 exons) than the previously published "normal" gene in this species -M- (812 nucleotides, 6 exons). This is the first report demonstrating the amelogenin alternative splicing in amphibian, revealing a unique exon 2b and two novel amelogenin gene transcripts in Plethodoncinereus.

SUBMITTER: Wang X 

PROVIDER: S-EPMC3694012 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Alternative Splicing of the Amelogenin Gene in a Caudate Amphibian, Plethodon cinereus.

Wang Xinping X   Xing Zeli Z   Zhang Xichen X   Zhu Lisai L   Diekwisch Thomas G H TG  

PloS one 20130626 6


As the major enamel matrix protein contributing to tooth development, amelogenin has been demonstrated to play a crucial role in tooth enamel formation. Previous studies have revealed amelogenin alternative splicing as a mechanism for amelogenin heterogeneous expression in mammals. While amelogenin and its splicing forms in mammalian vertebrates have been characterized, splicing variants of amelogenin gene still remains largely unknown in non-mammalian species. Here, using PCR and sequence analy  ...[more]

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