Integrin ?3 is overexpressed in glioma stem-like cells and promotes invasion.

ABSTRACT: Glioma stem-like cell (GSC) properties are responsible for gliomagenesis and recurrence. GSCs are invasive but its mechanism remains to be elucidated. Here, we attempted to identify the molecules that promote invasion in GSCs.Neurospheres and CD133? cells were collected from glioblastoma (GBM) specimens and glioma cell lines by sphere-formation method and magnetic affinity cell sorting, respectively. Differential expression of gene candidates, its role in invasion and its signaling pathway were evaluated in glioma cell lines.Neurospheres from surgical specimens attached to fibronectin and laminin, the receptors of which belong to the integrin family. Integrin ?3 was overexpressed in CD133? cells compared with CD133? cells in all the glioma cell lines (4 out of 4). Immunohistochemistry demonstrated the localisation of integrin ?3 in GBM cells, including invading cells, and in the tumour cells around the vessels, which is believed to be a stem cell niche. The expression of integrin ?3 was correlated with migration and invasion. The invasion activity of glioma cells was linked to the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2.Our results suggest that integrin ?3 contributes to the invasive nature of GSCs via ERK1/2, which renders integrin ?3 a prime candidate for anti-invasion therapy for GBM.

SUBMITTER: Nakada M 

PROVIDER: S-EPMC3694230 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature