Ontology highlight
ABSTRACT:
SUBMITTER: Shah P
PROVIDER: S-EPMC3694300 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Shah Premal P Ding Yang Y Niemczyk Malwina M Kudla Grzegorz G Plotkin Joshua B JB
Cell 20130601 7
Deep sequencing now provides detailed snapshots of ribosome occupancy on mRNAs. We leverage these data to parameterize a computational model of translation, keeping track of every ribosome, tRNA, and mRNA molecule in a yeast cell. We determine the parameter regimes in which fast initiation or high codon bias in a transgene increases protein yield and infer the initiation rates of endogenous Saccharomyces cerevisiae genes, which vary by several orders of magnitude and correlate with 5' mRNA foldi ...[more]