Unknown

Dataset Information

0

Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection.


ABSTRACT: Orthopoxviruses encode multiple proteins that modulate host immune responses. We determined whether cowpox virus (CPXV), a representative orthopoxvirus, modulated innate and acquired immune functions of human primary myeloid DCs and plasmacytoid DCs and monocyte-derived DCs (MDDCs). A CPXV infection of DCs at a multiplicity of infection of 10 was nonproductive, altered cellular morphology, and failed to reduce cell viability. A CPXV infection of DCs did not stimulate cytokine or chemokine secretion directly, but suppressed toll-like receptor (TLR) agonist-induced cytokine secretion and a DC-stimulated mixed leukocyte reaction (MLR). LPS-stimulated NF-?B nuclear translocation and host cytokine gene transcription were suppressed in CPXV-infected MDDCs. Early viral immunomodulatory genes were upregulated in MDDCs, consistent with early DC immunosuppression via synthesis of intracellular viral proteins. We conclude that a nonproductive CPXV infection suppressed DC immune function by synthesizing early intracellular viral proteins that suppressed DC signaling pathways.

SUBMITTER: Hansen SJ 

PROVIDER: S-EPMC3694803 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Cowpox virus inhibits human dendritic cell immune function by nonlethal, nonproductive infection.

Hansen Spencer J SJ   Rushton John J   Dekonenko Alexander A   Chand Hitendra S HS   Olson Gwyneth K GK   Hutt Julie A JA   Pickup David D   Lyons C Rick CR   Lipscomb Mary F MF  

Virology 20110221 2


Orthopoxviruses encode multiple proteins that modulate host immune responses. We determined whether cowpox virus (CPXV), a representative orthopoxvirus, modulated innate and acquired immune functions of human primary myeloid DCs and plasmacytoid DCs and monocyte-derived DCs (MDDCs). A CPXV infection of DCs at a multiplicity of infection of 10 was nonproductive, altered cellular morphology, and failed to reduce cell viability. A CPXV infection of DCs did not stimulate cytokine or chemokine secret  ...[more]

Similar Datasets

| S-EPMC8462324 | biostudies-literature
| PRJNA218164 | ENA
| PRJNA369073 | ENA
| S-EPMC6346447 | biostudies-literature
| S-EPMC5528940 | biostudies-other
| S-EPMC2570944 | biostudies-literature
| S-EPMC4766888 | biostudies-literature
| S-EPMC3219450 | biostudies-literature
| S-EPMC2655655 | biostudies-literature
| S-EPMC2791678 | biostudies-literature