Differential Inhibition of the TGF-? Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-? Receptor Type II.
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ABSTRACT:
SUBMITTER: Dituri F
PROVIDER: S-EPMC3694933 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
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Differential Inhibition of the TGF-β Signaling Pathway in HCC Cells Using the Small Molecule Inhibitor LY2157299 and the D10 Monoclonal Antibody against TGF-β Receptor Type II.
Dituri Francesco F Mazzocca Antonio A Fernando Joan J Peidrò Fernando Juan FJ Papappicco Patrizia P Fabregat Isabel I De Santis Flavia F Paradiso Angelo A Sabbà Carlo C Giannelli Gianluigi G
PloS one 20130627 6
We investigated blocking the TGF-β signaling pathway in HCC using two small molecule inhibitors (LY2157299, LY2109761) and a neutralizing humanized antibody (D10) against TGF-βRII. LY2157299 and LY2109761 inhibited HCC cell migration on Laminin-5, Fibronectin, Vitronectin, Fibrinogen and Collagen-I and de novo phosphorylation of pSMAD2. LY2157299 inhibited HCC migration and cell growth independently of the expression levels of TGF-βRII. In contrast to LY2157299, D10 showed a reduction in pSMAD2 ...[more]
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