Species identification of medically important trematodes in aquatic food samples using PCR-RFLP targeting 18S rRNA.

ABSTRACT: The authors describe a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method targeting the 18S rRNA gene for the species detection of medically important trematode infecting fish and oysters, and suggest that this PCR-RFLP method based on a specific Tre-18 primer and the restriction enzymes, Acc1, Ava2, Msp1, and Hinf1, is useful for the detection of parasites in aquatic food.

SUBMITTER: Pyo KH 

PROVIDER: S-EPMC3696915 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature