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Nicked-sleeve interface for two-dimensional capillary electrophoresis.

ABSTRACT: We report an improved interface for two-dimensional capillary electrophoresis. This interface is based on capillary tubing and a Plexiglas chip, both of which were milled using a micro-dicing saw. The interface was evaluated and compared to a traditional interface design for both pseudo one-dimensional and two-dimensional capillary electrophoresis. We observe less than 70% transfer efficiency for the traditional design and greater than 90% transfer efficiency with this new interface.

SUBMITTER: Flaherty RJ

PROVIDER: S-EPMC3697014 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Nicked-sleeve interface for two-dimensional capillary electrophoresis.

Flaherty Ryan J RJ Huge Bonnie J BJ Bruce Spencer M SM Dada Oluwatosin O OO Dovichi Norman J NJ

The Analyst 20130701 13

We report an improved interface for two-dimensional capillary electrophoresis. This interface is based on capillary tubing and a Plexiglas chip, both of which were milled using a micro-dicing saw. The interface was evaluated and compared to a traditional interface design for both pseudo one-dimensional and two-dimensional capillary electrophoresis. We observe less than 70% transfer efficiency for the traditional design and greater than 90% transfer efficiency with this new interface. ...[more]

PMID: 23702824

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