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Zirex: a novel zinc-regulated expression system for Lactococcus lactis.


ABSTRACT: Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.

SUBMITTER: Mu D 

PROVIDER: S-EPMC3697519 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Zirex: a novel zinc-regulated expression system for Lactococcus lactis.

Mu Dongdong D   Montalbán-López Manuel M   Masuda Yoshimitsu Y   Kuipers Oscar P OP  

Applied and environmental microbiology 20130510 14


Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes. ...[more]

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