Novel PCR-based methods enhance characterization of vaginal microbiota in a bacterial vaginosis patient before and after treatment.
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ABSTRACT:
SUBMITTER: Lambert JA
PROVIDER: S-EPMC3697572 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
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Novel PCR-based methods enhance characterization of vaginal microbiota in a bacterial vaginosis patient before and after treatment.
Lambert Janet A JA Kalra Apoorv A Dodge Cristina T CT John Susan S Sobel Jack D JD Akins Robert A RA
Applied and environmental microbiology 20130426 13
Deep characterization, even by next-generation sequencing, of the vaginal microbiota in healthy women or posttreatment bacterial vaginosis patients is limited by the dominance of lactobacilli. To improve detection, we offer two approaches: quantitative PCR (qPCR) using phylogenetic branch-inclusive primers and sequencing of broad-spectrum amplicons generated with oligomers that block amplification of lactobacilli. ...[more]
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