Ontology highlight
ABSTRACT:
SUBMITTER: Wu E
PROVIDER: S-EPMC3698950 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Wu Edlyn E Thivierge Caroline C Flamand Mathieu M Mathonnet Geraldine G Vashisht Ajay A AA Wohlschlegel James J Fabian Marc R MR Sonenberg Nahum N Duchaine Thomas F TF
Molecular cell 20101101 4
To understand how miRNA-mediated silencing impacts on embryonic mRNAs, we conducted a functional survey of abundant maternal and zygotic miRNA families in the C. elegans embryo. We show that the miR-35-42 and the miR-51-56 miRNA families define maternal and zygotic miRNA-induced silencing complexes (miRISCs), respectively, that share a large number of components. Using a cell-free C. elegans embryonic extract, we demonstrate that the miRISC directs the rapid deadenylation of reporter mRNAs with ...[more]