Unknown

Dataset Information

0

Involvement of microRNAs in infection of silkworm with bombyx mori cytoplasmic polyhedrosis virus (BmCPV).


ABSTRACT: Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the most important pathogens of silkworm. MicroRNAs (miRNAs) have been demonstrated to play key roles in regulating host-pathogen interaction. However, there are limited reports on the miRNAs expression profiles during insect pathogen challenges. In this study, four small RNA libraries from BmCPV-infected midgut of silkworm at 72 h post-inoculation and 96 h post-inoculation and their corresponding control midguts were constructed and deep sequenced. A total of 316 known miRNAs (including miRNA*) and 90 novel miRNAs were identified. Fifty-eight miRNAs displayed significant differential expression between the infected and normal midgut (P value < = 0.01 and fold change > = 2.0 or < = 0.5), among which ten differentially expressed miRNA were validated by qRT-PCR method. Further bioinformatics analysis of predicted target genes of differentially expressed miRNAs showed that the miRNA targets were involved in stimulus and immune system process in silkworm.

SUBMITTER: Wu P 

PROVIDER: S-EPMC3699532 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

altmetric image

Publications

Involvement of microRNAs in infection of silkworm with bombyx mori cytoplasmic polyhedrosis virus (BmCPV).

Wu Ping P   Han Shaohua S   Chen Tao T   Qin Guangxing G   Li Long L   Guo Xijie X  

PloS one 20130702 7


Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the most important pathogens of silkworm. MicroRNAs (miRNAs) have been demonstrated to play key roles in regulating host-pathogen interaction. However, there are limited reports on the miRNAs expression profiles during insect pathogen challenges. In this study, four small RNA libraries from BmCPV-infected midgut of silkworm at 72 h post-inoculation and 96 h post-inoculation and their corresponding control midguts were constructed and d  ...[more]

Similar Datasets

| S-EPMC5798952 | biostudies-literature
| S-EPMC113995 | biostudies-literature
| S-EPMC4376736 | biostudies-literature
| S-EPMC7173298 | biostudies-literature
| S-EPMC7428682 | biostudies-literature
| S-EPMC4645660 | biostudies-literature
| S-EPMC5795427 | biostudies-literature
| S-EPMC8073075 | biostudies-literature
| S-EPMC110377 | biostudies-literature