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Development and characterization of a chemically defined food for Drosophila.

ABSTRACT: Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Drosophila. We show that CDF can support the long-term culture of laboratory strains and demonstrate that this formulation has utility in isolating macronutrient from caloric density requirements in studies of development, longevity and reproduction.

SUBMITTER: Lee WC

PROVIDER: S-EPMC3699577 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Development and characterization of a chemically defined food for Drosophila.

Lee Wen-Chih WC Micchelli Craig A CA

PloS one 20130702 7

Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Droso ...[more]

PMID: 23844001

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