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A Biological Approach to the Synthesis of Silver Nanoparticles with Streptomyces sp JAR1 and its Antimicrobial Activity.

ABSTRACT: The biological approach to synthesize metal nanoparticles is an important aspect of current nanotechnology research. Silver nanoparticles have been well-known for their inhibitory and antimicrobial effects. The ever-increasing antibiotic resistance in pathogenic and opportunistic microorganisms is a major threat to the health care industry. In the present investigation, silver nanoparticles have been successfully biosynthesized by Streptomyces sp JAR1. Biosynthesized silver nanoparticles were characterized by means of several analytical techniques including a UV-Visible spectrophotometer, Fourier transform infrared spectroscopy, X-ray diffraction pattern analysis, and atomic force microscopy. An evaluation of the antimicrobial activity of silver nanoparticles (AgNPs) was carried out against clinically important pathogenic microorganisms. The metal nanoparticles were also evaluated for their combined effects with antibiotics against the clinical pathogens. The antibacterial activities of the antibiotics increased in the presence of the biologically synthesized AgNPs against the clinically important pathogens. The highest enhancing effect was observed for erythromycin against the test pathogens.

SUBMITTER: Chauhan R

PROVIDER: S-EPMC3700086 | biostudies-literature | 2013 Apr-Jun

REPOSITORIES: biostudies-literature

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A Biological Approach to the Synthesis of Silver Nanoparticles with Streptomyces sp JAR1 and its Antimicrobial Activity.

Chauhan Ritika R Kumar Abhishek A Abraham Jayanthi J

Scientia pharmaceutica 20130328 2

The biological approach to synthesize metal nanoparticles is an important aspect of current nanotechnology research. Silver nanoparticles have been well-known for their inhibitory and antimicrobial effects. The ever-increasing antibiotic resistance in pathogenic and opportunistic microorganisms is a major threat to the health care industry. In the present investigation, silver nanoparticles have been successfully biosynthesized by Streptomyces sp JAR1. Biosynthesized silver nanoparticles were ch ...[more]

PMID: 23833724

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Project description:BACKGROUND:Silver nanoparticles (AgNPs) are among the most effective antimicrobial agents that are used in the medicine and pharmaceutics. During the past decades, metal nanoparticles synthesis through application of the biological methods has increasingly been used, as the biologically synthesized particles are mostly non-toxic as well as effective. OBJECTIVES:The main goal for undertaking the present investigation was to evaluate the extracellular synthesis of the AgNPs by a native thermophilic Bacillus Sp. AZ1 that was isolated from a hot spring in Ardebil province. Subsequently the antimicrobial potentials of the nanoparticle was evaluated against several human pathogenic organisms. MATERIALS AND METHODS:The biosynthesized AgNPs were confirmed visually by appearance of a dark brown color formation in the mixture as well as silver surface plasmon resonance band by using UV-Visible spectroscopy. The AgNPs were further characterized by SEM, EDX and TEM. The antimicrobial activity of the AgNPs was investigated using Salmonella typhi, Escherichia coli, Staphylococcus epidermis, and Staphylococcus aureus, by applying disk diffusion method. RESULTS:Identification of the strain AZ1 by the 16S rRNA sequence analysis showed 99% sequence homology between this strain and B. licheniformis. The obtained UV-Visible spectrum of the aqueous medium containing silver ion, showed a peak at 425 nm which indicates a correspondence to the plasmon absorbance of the silver nanoparticles. The biosynthesized AgNPs were found to be in the size range of ~7-31 nm with spherical the shape. Studies regarding the antibacterial effect of the particles showed the highest inhibitory effect against the two strains; E. coli, and S. typhi, respectively. CONCLUSIONS:Our study presents a simple green synthesis process for the production of an extracellular nanoparticles which is environmental friendly. Biosynthesis of the AgNPs by a thermophilic bacillus from the hot spring (Qeynarjeh, Ardebil) in Iran with the highest similarity to Bacillus licheniformis is reported for the first time.

Dataset Information

A Biological Approach to the Synthesis of Silver Nanoparticles with Streptomyces sp JAR1 and its Antimicrobial Activity.

Publications

A Biological Approach to the Synthesis of Silver Nanoparticles with Streptomyces sp JAR1 and its Antimicrobial Activity.

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