Reverse genetics identifies the product of open reading frame 4 as an essential particle assembly factor of Nyamanini virus.
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ABSTRACT:
SUBMITTER: Herrel M
PROVIDER: S-EPMC3700212 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Publications
Reverse genetics identifies the product of open reading frame 4 as an essential particle assembly factor of Nyamanini virus.
Herrel Marieke M Haag Lars L Nilsson Josefina J Staeheli Peter P Schneider Urs U
Journal of virology 20130522 14
We established a reverse genetics system for Nyamanini virus (NYMV) and recovered green fluorescent protein (GFP)-expressing virus from full-length cDNA. Using this technology, we assessed the functions of two poorly characterized viral genes. NYMV lacking open reading frame 2 (ORF2) could not be rescued, whereas virus lacking ORF4 was replication competent. ORF4-deficient NYMV readily established a persisting noncytolytic infection but failed to produce infectious viral particles, supporting th ...[more]
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