Overlapping expression patterns and redundant roles for AP-2 transcription factors in the developing mammalian retina.

ABSTRACT: BACKGROUND:We have previously shown that the transcription factor AP-2? (Tcfap2a) is expressed in postmitotic developing amacrine cells in the mouse retina. Although retina-specific deletion of Tcfap2a did not affect retinogenesis, two other family members, AP-2? and AP-2?, showed expression patterns similar to AP-2?. RESULTS:Here we show that, in addition to their highly overlapping expression patterns in amacrine cells, AP-2? and AP-2? are also co-expressed in developing horizontal cells. AP-2? expression is restricted to amacrine cells, in a subset that is partially distinct from the AP-2?/?-immunopositive population. To address possible redundant roles for AP-2? and AP-2? during retinogenesis, Tcfap2a/b-deficient retinas were examined. These double mutants showed a striking loss of horizontal cells and an altered staining pattern in amacrine cells that were not detected upon deletion of either family member alone. CONCLUSIONS:These studies have uncovered critical roles for AP-2 activity in retinogenesis, delineating the overlapping expression patterns of Tcfap2a, Tcfap2b, and Tcfap2c in the neural retina, and revealing a redundant requirement for Tcfap2a and Tcfap2b in horizontal and amacrine cell development.

SUBMITTER: Bassett EA 

PROVIDER: S-EPMC3700368 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature