Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation.
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ABSTRACT:
SUBMITTER: Yim SA
PROVIDER: S-EPMC3701667 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation.
Yim Seung-Ae SA Lim Yun-Sook YS Kim Jong-Wook JW Hwang Soon B SB
PloS one 20130704 7
Hepatitis C virus (HCV) is highly dependent on cellular factors for its own propagation. By employing tandem affinity purification method, we identified pyruvate carboxylase (PC) as a cellular partner for NS5A protein. NS5A interacted with PC through the N-terminal region of NS5A and the biotin carboxylase domain of PC. PC expression was decreased in cells expressing NS5A and HCV-infected cells. Promoter activity of PC was also decreased by NS5A protein. However, FAS expression was increased in ...[more]
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