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Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging.

ABSTRACT: Fluorescence lifetime imaging (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. There has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed endoscopy systems. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of endoscopy systems that are suitable for in vivo tissue imaging. We present a compact wide-field time-gated FLIM flexible endoscope capable of continuous lifetime imaging of up to three fluorescence emission bands simultaneously. This endoscope design will facilitate the evaluation of FLIM for in vivo applications.

SUBMITTER: Cheng S

PROVIDER: S-EPMC3702045 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Flexible endoscope for continuous in vivo multispectral fluorescence lifetime imaging.

Cheng Shuna S Rico-Jimenez J Jesus JJ Jabbour Joey J Malik Bilal B Maitland Kristen C KC Wright John J Cheng Yi-Shing Lisa YS Jo Javier A JA

Optics letters 20130501 9

Fluorescence lifetime imaging (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. There has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed endoscopy systems. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of endoscopy systems that are ...[more]

PMID: 23632536

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