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Hyperosmotic stress induces cell cycle arrest in retinal pigmented epithelial cells.


ABSTRACT: Osmotic changes occur in many tissues and profoundly influence cell function. Herein, we investigated the effect of hyperosmotic stress on retinal pigmented epithelial (RPE) cells using a microarray approach. Upon 4-h exposure to 100 mM NaCl or 200 mM sucrose, 79 genes were downregulated and 72 upregulated. Three gene ontology categories were significantly modulated: cell proliferation, transcription from RNA polymerase II promoter and response to abiotic stimulus. Fluorescent-activated cell sorting analysis further demonstrated that owing to hyperosmotic stimulation for 24 h, cell count and cell proliferation, as well as the percentage of cells in G0/G1 and S phases were significantly decreased, whereas the percentage of cells in G2/M phases increased, and apoptosis and necrosis remained unaffected. Accordingly, hyperosmotic conditions induced a decrease of cyclin B1 and D1 expression, and an activation of the p38 mitogen-activated protein kinase. In conclusion, our results demonstrate that hypertonic conditions profoundly affect RPE cell gene transcription regulating cell proliferation by downregulation cyclin D1 and cyclin B1 protein expression.

SUBMITTER: Arsenijevic T 

PROVIDER: S-EPMC3702301 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Hyperosmotic stress induces cell cycle arrest in retinal pigmented epithelial cells.

Arsenijevic T T   Vujovic A A   Libert F F   Op de Beeck A A   Hébrant A A   Janssens S S   Grégoire F F   Lefort A A   Bolaky N N   Perret J J   Caspers L L   Willermain F F   Delporte C C  

Cell death & disease 20130606


Osmotic changes occur in many tissues and profoundly influence cell function. Herein, we investigated the effect of hyperosmotic stress on retinal pigmented epithelial (RPE) cells using a microarray approach. Upon 4-h exposure to 100 mM NaCl or 200 mM sucrose, 79 genes were downregulated and 72 upregulated. Three gene ontology categories were significantly modulated: cell proliferation, transcription from RNA polymerase II promoter and response to abiotic stimulus. Fluorescent-activated cell sor  ...[more]

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