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A repressor protein, Mnt, is a novel negative regulator of vascular smooth muscle cell hypertrophy by angiotensin II and neointimal hyperplasia by arterial injury.


ABSTRACT: The Max-interacting protein Mnt is a transcriptional repressor that can antagonize the transcriptional and proliferation-related activities of Myc. Here, we tested the hypothesis that Mnt is a negative regulator of pathological vascular remodeling.Adenovirus encoding Mnt or control GFP was infected to cultured rat vascular smooth muscle cells (VSMC) and carotid arteries after a balloon angioplasty.In VSMC, adenoviral gene transfer of Mnt suppressed angiotensin II-induced protein expression of early growth response protein-1 (Egr1) and its promoter activation. Mnt adenovirus did not interfere with upstream signaling of angiotensin II. Angiotensin II-induced protein accumulation in VSMC was inhibited by Mnt adenovirus. Mnt adenovirus also inhibited platelet-derived growth factor-induced VSMC proliferation. Moreover, Mnt adenovirus prevented neointima formation in response to arterial injury. The adenoviral Mnt gene transfer also prevented Egr1 induction in neointima.These data identify Mnt as a previously unrecognized negative regulator of pathological vascular remodeling.

SUBMITTER: Takayanagi T 

PROVIDER: S-EPMC3703465 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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A repressor protein, Mnt, is a novel negative regulator of vascular smooth muscle cell hypertrophy by angiotensin II and neointimal hyperplasia by arterial injury.

Takayanagi Takehiko T   Eguchi Akito A   Takaguri Akira A   Hinoki Akinari A   Bourne Allison M AM   Elliott Katherine J KJ   Hurlin Peter J PJ   Eguchi Satoru S  

Atherosclerosis 20130313 1


<h4>Objective</h4>The Max-interacting protein Mnt is a transcriptional repressor that can antagonize the transcriptional and proliferation-related activities of Myc. Here, we tested the hypothesis that Mnt is a negative regulator of pathological vascular remodeling.<h4>Methods</h4>Adenovirus encoding Mnt or control GFP was infected to cultured rat vascular smooth muscle cells (VSMC) and carotid arteries after a balloon angioplasty.<h4>Results</h4>In VSMC, adenoviral gene transfer of Mnt suppress  ...[more]

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