Dataset Information

Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4.

ABSTRACT:

Objective

To enhance the pigment production by Streptomyces sp. PM4 for evaluating its anticancer activity.

Methods

Response surface methodology was employed to enhance the production of red pigment from Streptomyces sp. PM4. Optimized pigment was purified and evaluated for the anticancer activity against HT1080, Hep2, HeLa and MCF7 cell lines by MTT assay.

Results

Based on the response surface methodology, it could be concluded that maltose (4.06 g), peptone (7.34 g), yeast extract (4.34 g) and tyrosine (2.89 g) were required for the maximum production of pigment (1.68 g/L) by the Streptomyces sp. PM4. Optimization of the medium with the above tested features increased the pigment yield by 4.6 fold. Pigment showed the potential anticancer activity against HT1080, HEp-2, HeLa and MCF-7 cell lines with the IC50 value of 18.5, 15.3, 9.6 and 8.5 respectively.

Conclusions

The study revealed that the maximum amount of pigment could be produced to treat cancer.

SUBMITTER: Karuppiah V

PROVIDER: S-EPMC3703560 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Publications

Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4.

Karuppiah Valliappan V Aarthi Chandramohan C Sivakumar Kannan K Kannan Lakshmanan L

Asian Pacific journal of tropical biomedicine 20130801 8

<h4>Objective</h4>To enhance the pigment production by Streptomyces sp. PM4 for evaluating its anticancer activity.<h4>Methods</h4>Response surface methodology was employed to enhance the production of red pigment from Streptomyces sp. PM4. Optimized pigment was purified and evaluated for the anticancer activity against HT1080, Hep2, HeLa and MCF7 cell lines by MTT assay.<h4>Results</h4>Based on the response surface methodology, it could be concluded that maltose (4.06 g), peptone (7.34 g), yeas ...[more]

PMID: 23905024

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Dataset Information

Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4.

Objective

Methods

Results

Conclusions

Publications

Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4.

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