Ontology highlight
ABSTRACT:
SUBMITTER: Thievessen I
PROVIDER: S-EPMC3704983 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Thievessen Ingo I Thompson Peter M PM Thompson Peter M PM Berlemont Sylvain S Plevock Karen M KM Plotnikov Sergey V SV Zemljic-Harpf Alice A Ross Robert S RS Davidson Michael W MW Danuser Gaudenz G Campbell Sharon L SL Waterman Clare M CM
The Journal of cell biology 20130701 1
In migrating cells, integrin-based focal adhesions (FAs) assemble in protruding lamellipodia in association with rapid filamentous actin (F-actin) assembly and retrograde flow. How dynamic F-actin is coupled to FA is not known. We analyzed the role of vinculin in integrating F-actin and FA dynamics by vinculin gene disruption in primary fibroblasts. Vinculin slowed F-actin flow in maturing FA to establish a lamellipodium-lamellum border and generate high extracellular matrix (ECM) traction force ...[more]