Ontology highlight
ABSTRACT:
SUBMITTER: Garcia-Fernandez E
PROVIDER: S-EPMC3706556 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
García-Fernández Esther E Frank Daniel J DJ Frank Daniel J DJ Galán Beatriz B Kells Petrea M PM Podust Larissa M LM García José L JL Ortiz de Montellano Paul R PR
Environmental microbiology 20130314 8
Degradation of the cholesterol side-chain in Mycobacterium tuberculosis is initiated by two cytochromes P450, CYP125A1 and CYP142A1, that sequentially oxidize C26 to the alcohol, aldehyde and acid metabolites. Here we report characterization of the homologous enzymes CYP125A3 and CYP142A2 from Mycobacterium smegmatis mc(2) 155. Heterologously expressed, purified CYP125A3 and CYP142A2 bound cholesterol, 4-cholesten-3-one, and antifungal azole drugs. CYP125A3 or CYP142A2 reconstituted with spinach ...[more]