Ontology highlight
ABSTRACT:
SUBMITTER: Sengupta RN
PROVIDER: S-EPMC3707313 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Sengupta Raghuvir N RN Herschlag Daniel D Piccirilli Joseph A JA
ACS chemical biology 20111104 2
Protein and RNA enzymes that catalyze phosphoryl transfer reactions frequently contain active site metal ions that interact with the nucleophile and leaving group. Mechanistic models generally hinge upon the assumption that the metal ions stabilize negative charge buildup along the reaction coordinate. However, experimental data that test this assumption directly remain difficult to acquire. We have used an RNA substrate bearing a 3'-thiol group to investigate the energetics of a metal ion inter ...[more]