Ontology highlight
ABSTRACT:
SUBMITTER: Fischer M
PROVIDER: S-EPMC3708723 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Molecular biology of the cell 20130515 14
Oxidation of cysteine residues to disulfides drives import of many proteins into the intermembrane space of mitochondria. Recent studies in yeast unraveled the basic principles of mitochondrial protein oxidation, but the kinetics under physiological conditions is unknown. We developed assays to follow protein oxidation in living mammalian cells, which reveal that import and oxidative folding of proteins are kinetically and functionally coupled and depend on the oxidoreductase Mia40, the sulfhydr ...[more]