Dataset Information

Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples.

ABSTRACT: A polyphasic analysis was undertaken of seven independent isolates of gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7-100?%, and the closest species with a validly published name was Neisseria lactamica (96.9?% similarity to the type strain). DNA-DNA hybridization confirmed that these isolates are of the same species and are distinct from their nearest phylogenetic neighbour, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA gene sequences indicated that the novel species belongs in the genus Neisseria. The predominant cellular fatty acids were C16?:?0, summed feature 3 (C16?:?1?7c and/or iso-C15?:?0 2-OH) and C18?:?1?7c. The cellular fatty acid profile, together with other phenotypic characters, further supports the inclusion of the novel species in the genus Neisseria. The name Neisseria oralis sp. nov. (type strain 6332(T) ?=?DSM 25276(T) ?=?LMG 26725(T)) is proposed.

SUBMITTER: Wolfgang WJ

PROVIDER: S-EPMC3709538 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples.

Wolfgang William J WJ Passaretti Teresa V TV Jose Reashma R Cole Jocelyn J Coorevits An A Carpenter Andrea N AN Jose Sherly S Van Landschoot Anita A Izard Jacques J Kohlerschmidt Donna J DJ Vandamme Peter P Dewhirst Floyd E FE Fisher Mark A MA Musser Kimberlee A KA

International journal of systematic and evolutionary microbiology 20120713 Pt 4

A polyphasic analysis was undertaken of seven independent isolates of gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7-100 %, and the closest species with a validly published name was Neisseria lactamica (96.9 % similarity to the type strain). DNA-DNA hybridization confirmed that these isolates are of th ...[more]

PMID: 22798652

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Project description:Mucormycosis is known to be a rare opportunistic infection caused by fungal organisms belonging to the Mucorales order, which includes the Syncephalastrum species. These moulds are rarely involved in clinical diseases and are generally seen as contaminants in clinical laboratories. However, in recent years, case reports of human infections due to Syncephalastrum have increased, especially in immunocompromised hosts. In this study, we described two new Syncephalastrum species, which were isolated from human nails and sputum samples from two different patients. We used several methods for genomic and phenotypic characterisation. The phenotypic analysis relied on the morphological features, analysed both by optical and scanning electron microscopy. We used matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, energy-dispersive X-ray spectroscopy, and BiologTM technology to characterise the proteomic, chemical mapping, and carbon source assimilation profiles, respectively. The genomic analysis relied on a multilocus DNA sequence analysis of the rRNA internal transcribed spacers and D1/D2 large subunit domains, fragments of the translation elongation factor-1 alpha, and the β-tubulin genes. The two novel species in the genus Syncephalastrum, namely S. massiliense PMMF0073 and S. timoneanum PMMF0107, presented a similar morphology: irregular branched and aseptate hyphae with ribbon-like aspects and terminal vesicles at the apices all surrounded by cylindrical merosporangia. However, each species displayed distinct phenotypic and genotypic features. For example, S. timoneanum PMMF0107 was able to assimilate more carbon sources than S. massiliense PMMF0073, such as adonitol, α-methyl-D-glucoside, trehalose, turanose, succinic acid mono-methyl ester, and alaninamide. The polyphasic approach, combining the results of complementary phenotypic and genomic assays, was instrumental for describing and characterising these two new Syncephalastrum species.

Dataset Information

Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples.

Publications

Neisseria oralis sp. nov., isolated from healthy gingival plaque and clinical samples.

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