SUMOylation alters CRMP2 regulation of calcium influx in sensory neurons.
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ABSTRACT:
SUBMITTER: Ju W
PROVIDER: S-EPMC3710342 | biostudies-literature | 2013 May-Jun
REPOSITORIES: biostudies-literature
Publications
SUMOylation alters CRMP2 regulation of calcium influx in sensory neurons.
Ju Weina W Li Qi Q Wilson Sarah M SM Brittain Joel M JM Meroueh Louay L Khanna Rajesh R
Channels (Austin, Tex.) 20130319 3
The axon/dendrite specification collapsin response mediator protein 2 (CRMP2) bidirectionally modulates N-type voltage-gated Ca ( 2+) channels (CaV2.2). Here we demonstrate that small ubiquitin-like modifier (SUMO) protein modifies CRMP2 via the SUMO E2-conjugating enzyme Ubc9 in vivo. Removal of a SUMO conjugation site KMD in CRMP2 (K374A/M375A/D376A; CRMP2AAA) resulted in loss of SUMOylated CRMP2 without compromising neurite branching, a canonical hallmark of CRMP2 function. Increasing SUMOyla ...[more]
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