Efficient experimental design and analysis of real-time PCR assays.
Ontology highlight
ABSTRACT:
SUBMITTER: Hui K
PROVIDER: S-EPMC3710343 | biostudies-literature | 2013 May-Jun
REPOSITORIES: biostudies-literature
Publications
Efficient experimental design and analysis of real-time PCR assays.
Hui Kwokyin K Feng Zhong-Ping ZP
Channels (Austin, Tex.) 20130319 3
Real-time polymerase chain reaction (qPCR) is currently the standard for gene quantification studies and has been extensively used in large-scale basic and clinical research. The operational costs and technical errors can become a significant issue due to the large number of sample reactions. In this paper, we present an experimental design strategy and an analysis procedure that are more efficient requiring fewer sample reactions than the traditional approach. We verified mathematically and exp ...[more]
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