Unknown

Dataset Information

0

Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States.


ABSTRACT: European-like field isolates of porcine reproductive and respiratory syndrome virus (PRRSV) have recently emerged in North America. The full-length genomic sequence of an index isolate characterized in 1999, strain EuroPRRSV, served as the reference strain for further studies of the evolution and epidemiology of European-like isolates (type 1) in the United States. Strain EuroPRRSV shared 90.1 to 100% amino acid identity with the prototype European strain, Lelystad, within the structural and nonstructural open reading frames (ORFs) and 95.3% overall nucleotide identity. The 5' untranslated region and two nonstructural regions within ORF 1 were closely examined due to significant divergence from strain Lelystad. A 51-bp deletion in a region within ORF 1a, coding for nonstructural protein 2 (NSP2), was observed. Sequence analysis of the structural ORFs 2 to 7 of additional European-like isolates indicated that these isolates share 93% nucleotide identity with one another and 95 to 96% identity with the Lelystad strain but only 70% identity with the North American reference strain VR-2332. Phylogenetic analysis with published PRRSV ORF 3, 5, and 7 nucleotide sequences indicated that these newly emerging isolates form a clade with the Lelystad and United Kingdom PRRSV isolates. Detailed analysis of four of these isolates with a panel of 60 monoclonal antibodies directed against the structural proteins confirmed a recognition pattern that was more consistent with strain Lelystad than with other North American isolates.

SUBMITTER: Ropp SL 

PROVIDER: S-EPMC371078 | biostudies-literature | 2004 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States.

Ropp Susan L SL   Wees Carrie E Mahlum CE   Fang Ying Y   Nelson Eric A EA   Rossow Kurt D KD   Bien Melissa M   Arndt Bill B   Preszler Sarah S   Steen Pamela P   Christopher-Hennings Jane J   Collins James E JE   Benfield David A DA   Faaberg Kay S KS  

Journal of virology 20040401 7


European-like field isolates of porcine reproductive and respiratory syndrome virus (PRRSV) have recently emerged in North America. The full-length genomic sequence of an index isolate characterized in 1999, strain EuroPRRSV, served as the reference strain for further studies of the evolution and epidemiology of European-like isolates (type 1) in the United States. Strain EuroPRRSV shared 90.1 to 100% amino acid identity with the prototype European strain, Lelystad, within the structural and non  ...[more]

Similar Datasets

| S-EPMC9730796 | biostudies-literature
| S-EPMC5543689 | biostudies-literature
| S-EPMC3472791 | biostudies-literature
| S-EPMC7111980 | biostudies-literature
| S-EPMC5489505 | biostudies-literature
| S-EPMC8558496 | biostudies-literature
| S-EPMC2898778 | biostudies-literature
| S-EPMC5525233 | biostudies-literature
| S-EPMC4974319 | biostudies-literature
| S-EPMC4709888 | biostudies-literature