Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy.

ABSTRACT: Synthesis of new proteins, a key step in the central dogma of molecular biology, has been a major biological process by which cells respond rapidly to environmental cues in both physiological and pathological conditions. However, the selective visualization of a newly synthesized proteome in living systems with subcellular resolution has proven to be rather challenging, despite the extensive efforts along the lines of fluorescence staining, autoradiography, and mass spectrometry. Herein, we report an imaging technique to visualize nascent proteins by harnessing the emerging stimulated Raman scattering (SRS) microscopy coupled with metabolic incorporation of deuterium-labeled amino acids. As a first demonstration, we imaged newly synthesized proteins in live mammalian cells with high spatia

SUBMITTER: Wei L 

PROVIDER: S-EPMC3710790 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature