Ontology highlight
ABSTRACT:
SUBMITTER: Zelensky AN
PROVIDER: S-EPMC3711438 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Zelensky Alex N AN Sanchez Humberto H Ristic Dejan D Vidic Iztok I van Rossum-Fikkert Sari E SE Essers Jeroen J Wyman Claire C Kanaar Roland R
Nucleic acids research 20130510 13
Caffeine is a widely used inhibitor of the protein kinases that play a central role in the DNA damage response. We used chemical inhibitors and genetically deficient mouse embryonic stem cell lines to study the role of DNA damage response in stable integration of the transfected DNA and found that caffeine rapidly, efficiently and reversibly inhibited homologous integration of the transfected DNA as measured by several homologous recombination-mediated gene-targeting assays. Biochemical and stru ...[more]